Masters, J. Book Google Scholar. Human cancer cell lines: fact and fantasy. Nature Rev. Cell Biol. Wistuba, I. Comparison of features of human breast cancer cell lines and their corresponding tumours. Sanford, K. A quest for the mechanism of 'spontaneous' malignant transformation in culture with associated advances in culture technology.
Cancer 82 , — Macville, M. Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Scherer, W. Studies on the propagation in vitro of poliomyelitis viruses. Brown, R. The mass production and distribution of HeLa cells at Tuskegee Institute, — Allied Sci. Arlett, C. The use of dubious cell lines in research: is trust enough? Lancet Oncol. Rothfels, K. Begg, R. Gartler, S. Genetic markers as tracers in cell culture. Apparent HeLa cell contamination of human heteroploid cell lines.
Nature , — Nelson-Rees, W. Banded marker chromosomes as indicators of intraspecies cellular contamination. Science , HeLa cultures defined.
Science , 96—98 Cross-contamination of cells in culture. Science , — Responsibility for truth in research. B , — Responsibility for trust in research. Markovic, O. Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell Dev. MacLeod, R. Widespread intraspecies cross-contamination of human tumour cell lines.
Hirzel said. I thought having our product lines gave us greater cause and reason for doing something. It has to come from a visceral desire to contribute and make an impact. At the Reck-Peterson lab, the scientists saw a direct connection between what happened to Henrietta Lacks and to current demonstrations and struggles for racial and social justice. Stevens said. How should patients benefit, if at all, from research using their cells and tissues?
Join the conversation below. Reck-Peterson and her team considered several options, including using other cell lines or making monetary donations. They ended up speaking with members of the Lacks family, who told them the family wanted scientists to continue to use the cells to benefit science.
Skloot, the author, said that when she initially set up the foundation, she hoped businesses and research institutions that rely so heavily on HeLa cells would donate to help the family. But donations have mainly come from individuals rather than corporations or institutions. She said that the Lacks family is large and that different members hold various viewpoints on what should be done to rectify past injustices and how to move forward in a way that also ensures that future patients are protected.
Write to Amy Dockser Marcus at amy. Gey later propagated the cells to create the HeLa cell line and made them freely available to other researchers. The cells were later commercialised but have never been patented. While the multibillion-dollar biotech industry was built on the back of the HeLa cells, her descendants received no financial compensation and were not consulted in the projects in which they were used.
In August last year, UK based firm Abcam became the first ever biotechnology company to make a donation to the Henrietta Lacks Foundation that has used HeLa cells in its research. In the s, a leukaemia patient named John Moore donated blood samples believing they would be used for diagnostic purposes. Instead, the material was cultured into a cell line that became part of a patent application.
The HeLa cell line gave them the time and the possibility to conduct repeatable experiments on human cells, without testing directly on humans.
And to this day, HeLa cells have saved countless lives, and many scientific landmarks such as cloning, gene mapping, in vitro fertilization, the polio vaccine… have used HeLa cells and owe everything to the life and death of Henrietta Lacks.
You can find more information about Henrietta Lacks, her story, her legacy, and bioethical standards here. With all these characteristics, HeLa cells became progressively popular cellular models for life scientists willing to study mechanism of action of diseases or therapeutically active drug molecules. They have also been used to decipher cell signalling events such as DNA damage repair ref. Recently, HeLa cells have been used to develop cellular models in which a specific gene of interest is silenced by genome editing.
Several methods are available for gene editing ex. This technology has already been validated in numerous scientific articles covering various biological domains and applications such as DNA Repair, Epigenetics, Ubiquitination and the cell cycle, Drug discovery ex. To know more about these SilenciX HeLa cells stably silenced please browe the technical info page, HeLa cells stably silenced: SilenciX KD cell lines , that describes the principle of the KD, the list of catalog SilenciX cell lines together with numerous scientific publications using this SilenciX technology.
Dear Joshua, Thank you for your nice comment. It was actually written by an ex colleague of mine.
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